HumanKine® recombinant human IL-7 protein
– GMP grade
(Cat No. HZ-1281-GMP)

HEK293 expressed, Endotoxin-free, Animal-component free

Size：10 μg, 100 μg, 1000 μg, Custom

Synonyms：

IL 7, IL7, IL-7, interleukin 7

Technical Specifications：

GeneID	3574
Species	Human
Expression	HEK293
EC50	0.1-1.4 ng/mL
Specific Activity	minimally 7.00 x 10^7 IU/mg,  typically 1.93 x 10^8 IU/mg
Purity	>95%
Endotoxin	<1 EU/µg
Accession Number	P13232
Molecular Mass	19, 25, and 30 kDa reduced and non-reduced, monomer, glycosylated
Formulation	1x PBS, See Certificate of Analysis for details
Species Reactivity	human, mouse

Validation Data Gallery：

Purity of GMP-grade recombinant human IL-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP recombinant human IL-7 (HZ-1281-GMP) stimulates dose-dependent proliferation of the 2E8 murine B lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.1-1.4 ng/mL.

 

Stability and Reconstitution：

Stability & Storage	Product Form	Temperature Conditions	Storage Time
	Lyophilized	-20°C to -80°C	Until Expiry Date
		Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
		4°C	1 week
	Avoid repeated freeze-thaw cycles.
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

 

Background Information：

Interleukin-7 (IL-7, 白血球介素-7) 是一種參與 B 細胞和 T 細胞發育的關鍵細胞激素。它在成熟 T 淋巴細胞的發育、存活、維持和恢復穩態中擔任重要角色，並且是 B 細胞發育過程中分化、存活、增殖和成熟的關鍵調節因子。此外，IL-7 可增強自然殺手 (NK) 細胞的抗病毒功能和擴增，並調節樹突狀細胞的發育和分化。據報導，IL-7 也參與中樞神經系統發育、肌肉生成和骨骼肌細胞遷移的調節（PMID：29663382；29655570；29449560）。

GMP Quality Policies：

HumanKine® GMP (Good Manufacturing Practice) 重組蛋白的生產和驗證均符合 ISO 13485 品質管理體系，並符合 GMP 標準。
我們的 GMP 重組蛋白 不含動物源成分 (animal component free, ACF)、不含異種成分 (xeno free, XF) 且不含標籤 (tag free, TF)。
按此了解更多相關資訊。

Datasheet：

HumanKine® recombinant human IL-7 protein- GMP grade(Cat No. HZ-1281-GMP) datasheet

資料來源：

Proteintech：HumanKine® recombinant human IL-7 protein- GMP grade(Cat No. HZ-1281-GMP)

 

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