HumanKine® recombinant human IL-2 protein
– GMP grade
(Cat No. HZ-1015-GMP)

HEK293 expressed, Endotoxin-free, Animal-component free

Size：10 μg, 100 μg, 1000 μg, Custom

Technical Specifications：

GeneID	3558
Species	Human
Expression	HEK293
EC50	0.05-0.35 ng/mL
Specific Activity	minimally 1.7 x 10^6 IU/mg  Typically 8.63 x 106 IU/mg
Purity	>95%
Endotoxin	<0.1 EU/µg
Accession Number	P60568
Molecular Mass	17 kDa reduced,16 kDa non-reduced, monomer, glycosylated
Formulation	1x PBS, See Certificate of Analysis for details
Species Reactivity	human, mouse

Validation Data Gallery：

Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-2 (HZ-1015-GMP) induces dose-dependent proliferation of the CTLL2 (mouse cytotoxic T cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. CTLL2 cells were treated with increasing concentrations of GMP recombinant IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.05-0.35 ng/mL.

 

Stability and Reconstitution：

Stability & Storage	Product Form	Temperature Conditions	Storage Time
	Lyophilized	-20°C to -80°C	Until Expiry Date
		Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
		4°C	1 week
	Avoid repeated freeze-thaw cycles.
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

 

Background Information：

Interleukin-2 (IL-2, 白血球介素-2) 是一種和T細胞相關的關鍵細胞激素，可作為多種白血球的生長因子。在免疫系統中扮演核心角色，負責促進 T 細胞的增殖、活化，並調節其他免疫細胞（如 B 細胞、NK 細胞）的活性，以完成免疫反應。
IL-2對於維持免疫穩定、T regulatory cell的正常功能和自體耐受性至關重要。它可調節免疫細胞恆定，並已被用於治療包括癌症和自體免疫疾病在內的多種疾病。 IL-2透過IL-2Rβ和IL-2Rγreceptor subunits的異二聚化(heterodimerization)進行訊號傳導（PMID：25992858、24907378、15034008、17981641）。

GMP Quality Policies：

HumanKine® GMP (Good Manufacturing Practice) 重組蛋白的生產和驗證均符合 ISO 13485 品質管理體系，並符合 GMP 標準。
我們的 GMP 重組蛋白 不含動物源成分 (animal component free, ACF)、不含異種成分 (xeno free, XF) 且不含標籤 (tag free, TF)。
按此了解更多相關資訊。

Datasheet：

HumanKine® recombinant human IL-2 protein- GMP grade(Cat No. HZ-1015-GMP) datasheet

資料來源：

Proteintech：HumanKine® recombinant human IL-2 protein- GMP grade(Cat No. HZ-1015-GMP)

 

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